pY Pichia Secretory SUMOstar Expression Kit
Product Sizes
500 reactions
PY-2160-1000-500REACTIONS
About this Product
- SKU:
- PY-2160-1000
- Extra Details:
- SUMOstar Fusion Protein Technology \nThe pY-secSUMOstar vector enables expression and secretion of SUMOstar fusions in Pichia pastoris. Protein expression is driven by the primary alcohol oxidase (AOX1) promoter, which is both tightly regulated and highly inducible. The alpha mating factor (S. cerevisiae) targets the fusion protein to the secretory pathway, while both a FLAG and a His6 tag preceed SUMOstar for convenient purification. Chromosomal integration is accomplished by DNA linearization within the promoter using a variety of convenient endonuclease restriction sites, while mult-copy integrants can be obtained through strain selection against increasing concentrations of the antibiotic Zeocin. \nComponents: \n-- pY-secSUMOstar vector (Cat.# 2161) - 20 ug \n-- SUMOstar Protease (Cat.# 4110) - 500 Units \n-- SUMOstar Protease control protein (Cat.# 5010) - 100 ug \n-- Anti-SUMOstar antibody (Cat.# AB7002) - 50 ug
- Shipping Conditions:
- Dry Ice
- Storage Conditions:
- -70[o]C
- Supplier:
- LifeSensors
- Type:
- Assay Kits: Protein Manufacture
