HighYield T7 Fluorescein RNA Labeling Kit (UTP-based), Preparation of randomly Fluorescein-modified RNA probes by in vitro transcription with Fluorescein-12-UTP
Product Sizes
20 reactions
£448.00
RNT-101-FAMX-20REACTIONS
About this Product
- SKU:
- RNT-101-FAMX
- Conjugate:
- FITC
- Extra Details:
- HighYield T7 Fluorescein RNA Labeling Kit is designed to produce randomly Fluorescein-modified RNA probes via in vitro transcription. Such probes are ideally suited for in situ hybridization and Northern Blot experiments. The labeling principle is similar to the underlying labeling principles of Fluorescein RNA Labeling Mix (Roche). Fluorescein-12-UTP is efficiently incorporated into RNA as substitute for its natural counterpart UTP using an optimized reaction buffer and T7 RNA Labeling Polymerase Mix. 35 % Fluorescein-12-UTP substitution typically results in an optimal balance between reaction and labeling efficiency. Individual optimization of Fluorescein-12-UTP/UTP ratio however, can easily be achieved with the single nucleotide format. The resulting Fluorescein-modified RNA probe can subsequently be detected by fluorescence spectroscopy. The kit contains sufficient reagents for 20 labeling reactions of 20 ul each (35 % Fluorescein-12-UTP substitution, 2.5 mM ATP, GTP, CTP, 0.2 mM UTP, 0.1 mM Fluorescein-12-UTP).
- Shipping Conditions:
- Blue Ice
- Storage Conditions:
- -20[o]C Aliquot. Avoid freeze/thaw cycles.
- Supplier:
- Jena Bioscience
- Type:
- Assay Kits: Labelling
