HighYield T7 Cy3 RNA Labeling Kit (UTP-based), Preparation of randomly Cy3-modified RNA probes by in vitro transcription with UTP-X-CY3
Product Sizes
20 reactions
RNT-101-CY3-20REACTIONS
About this Product
- SKU:
- RNT-101-CY3
- Conjugate:
- Cyanine 3
- Extra Details:
- HighYield T7 Cy3 RNA Labeling Kit is designed to produce randomly Cy3-modified RNA probes via in vitro transcription. Such probes are ideally suited for in situ hybridization and Northern Blot experiments. UTP-X-CY3 (also known as Cy3-17-UTP from GE Healthcare) is efficiently incorporated into RNA as substitute for its natural counterpart UTP using an optimized reaction buffer and T7 RNA Labeling Polymerase Mix. 35 % UTP-X-Cy3 substitution typically results in an optimal balance between reaction and labeling efficiency. Individual optimization of UTP-X-Cy3/UTP ratio however, can easily be achieved with the single nucleotide format. The resulting Cy3-modified RNA probe can subsequently be detected by fluorescence spectroscopy. The kit contains sufficient reagents for 20 labeling reactions of 20 ul each (35 % UTP-X-Cy3 substitution, 2.5 mM ATP, GTP, CTP, 0.2 mM UTP, 0.1 mM UTP-X-Cy3).
- Shipping Conditions:
- Blue Ice
- Storage Conditions:
- -20[o]C Aliquot. Avoid freeze/thaw cycles.
- Supplier:
- Jena Bioscience
- Type:
- Assay Kits: Labelling
