HighYield T7 Azide RNA Labeling Kit (UTP-based), Preparation of randomly Azide-modified RNA probes by in vitro transcription with 5-Azido-C3-UTP
Product Sizes
25 reactions
RNT-101-AZ-25REACTIONS
About this Product
- SKU:
- RNT-101-AZ
- Extra Details:
- HighYield T7 Azide RNA Labeling Kit is designed to produce randomly Azide-modified RNA probes via in vitro transcription. Such probes are ideally suited for in situ hybridization, Northern Blot or crosslinking experiments. 5-Azido-C3-UTP is efficiently incorporated into RNA as substitute for its natural counterpart UTP using an optimized reaction buffer and T7 RNA Labeling Polymerase Mix. 35 % 5-Azido-C3-UTP substitution typically results in an optimal balance between reaction and labeling efficiency. Individual optimization of 5-Azido-C3-UTP/UTP ratio however, can easily be achieved with the single nucleotide format. The resulting Azide-modified RNA probe can subsequently be labeled by copper-free Click Chemistry with Dibenzocyclooctyne (DBCO)-functionalized detection reagents (e.g DBCO-functionalized fluorescent dyes or (Desthio)Biotin) thereby forming a stable, covalently labeled RNA probe[1]. The kit contains sufficient reagents for 25 labeling reactions of 20 ul each (35 % 5-Azido-C3-UTP substitution, 1 mM ATP, GTP, CTP, 0.65 mM UTP, 0.35 mM 5-Azido-C3-UTP).
- Shipping Conditions:
- Blue Ice
- Storage Conditions:
- -20[o]C Aliquot. Avoid freeze/thaw cycles.
- Supplier:
- Jena Bioscience
- Type:
- Assay Kits: Labelling
