T7 P&L RNA Polymerase HC, Highly concentrated and modified T7 RNA Polymerase for set-up of high yield in vitro transcription reactions
Product Sizes
50 ul
£424.00
RNT-018-50UL
About this Product
- SKU:
- RNT-018
- Concentration:
- 2-2.2 ug/ul
- Extra Details:
- Bacteriophage T7 RNA Polymerase is a DNA-dependent RNA polymerase (99 kDa) that catalyzes in vitro RNA synthesis from a DNA sequence containing a T7 phage promoter. The highly concentrated version and modified T7 RNA Polymerase HC is ideally suited for set-up and optimization of high yield in vitro transcription reactions. Proline 266 has been replaced by leucine (P266L) which has been associated with decreased abortive transcription[1].increased 5' homogeneity of transcripts synthesized from A-initiating phi2.5 promoter[2]increased 5' incorporation efficiency of GTP analogs[3] The provided T7 IVT Set-up Buffer, MgCl2 and DTT solutions enable testing of different reaction conditions such as nucleotide/ MgCl2 concentrations. Recommendation for assay set-up with minimal optimization: HighYield T7 P&L RNA Synthesis Kit (#RNT-201). Activity: 1 ul T7 P&L RNA Polymerase HC generates >/= 30 ug RNA in 10 min at 37°. This is comparable to 1000 U of NEB's T7 RNA Polymerase (High Concentration), #M0460T (activity assay conditions: 1x T7 IVT Set-up Buffer supplemented with 10 mM DTT, 12 mM MgCl2, 3 mM NTPs, 1 ul T7 RNA Polymerase HC and 1 ug DNA template (1.4 kpb RNA transcript) in 20 ul. RNA yield was determined with a fluorescence microplate assay.)
- Physical State:
- Liquid
- Purity:
- ≥95%
- Shipping Conditions:
- Blue Ice
- Storage Conditions:
- -20[o]C Aliquot. Avoid freeze/thaw cycles.
- Supplier:
- Jena Bioscience
- Type:
- Proteins, Peptides, Small Molecules & Other Biomolecules: Enzymes
