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Aminoallyl-dUTP-5/6-TAMRA, Tetramethyl-Rhodamine-5-dUTP

Product Sizes
30 ul
£339.00
NU-803-TAM-30UL
About this Product
SKU:
NU-803-TAM
CE/IVD:
RUO
Conjugate:
TRITC
Concentration:
1.0-1.1 mM
Extra Details:
Aminoallyl-dUTP-5/6-TAMRA is recommended for direct enzymatic labeling of DNA/cDNA by Nick Translation. It is incorporated as substitute for its natural counterpart dTTP. The resulting Dye-labeled DNA/cDNA probes are ideally suited for fluorescence hybridization applications such as FISH or microarray-based gene expression profiling. Optimal substrate properties and thus labeling efficiency is ensured by an optimized linker attached to the C5 position of uridine. Recommended Aminoallyl-dUTP-5/6-TAMRA/dTTP ratio for Nick Translation: 35% Aminoallyl-dUTP-5/6-TAMRA/ 65% dTTP Please note: Protect the Dye-labeled dUTP from exposure to light and carry out experimental procedures in low light conditions. The optimal final concentration of the Dye-labeled dUTP may very depending on the application and assay conditions. For optimal produdct yields and high incorporation rates an individual optimization of the Dye-labeled-dUTP/dTTP ratio is recommended.
Molecular Weight:
935.66 g/mol (free acid)
Physical State:
Solution
Purity:
≥95%
Shipping Conditions:
Blue Ice
Storage Conditions:
RT, 2-8[o]C
Supplier:
Jena Bioscience
Type:
Molecular Biology Reagents: NGS Reagents