DIG-11-ddUTP
Product Sizes
25 ul
£245.00
NU-1619-DIGX-25UL
About this Product
- SKU:
- NU-1619-DIGX
- CE/IVD:
- RUO
- Concentration:
- 1.0-1.1 mM
- Extra Details:
- Digoxigenin-11-ddUTP is enzymatically incorporated into the 3'-End of oligonucleotides. It acts as a chain terminator leading to the addition of one Digoxigenin label per molecule only. The resulting Digoxigenin-labeled oligonucleotide probes are subsequently detected using Digoxigenin-antibodies conjugated with horseradish peroxidase (HRP), alkaline phosphatase (AP) or a fluorescent dye. Optimal substrate properties and thus labeling efficiency is ensured by a 11-atom linker attached to the C5 position of uridine. Recommended molar ratio of Digoxigenin-11-ddUTP to free 3'-OH groups: 10:1. Please note: The optimal final concentration and molar ratio of Digoxigenin-11-ddUTP to free 3'-OH groups may very depending on the application and assay conditions. For optimal incorporation rates an individual optimization of the Digoxigenin-11-ddUTP concentration and molar ratio is recommended.
- Molecular Weight:
- 1048.91 g/mol (free acid)
- Physical State:
- Solution
- Purity:
- ≥95%
- Shipping Conditions:
- Blue Ice
- Storage Conditions:
- RT, 2-8[o]C
- Supplier:
- Jena Bioscience
- Type:
- Molecular Biology Reagents: NGS Reagents
