HighFidelity Biotin16 PCR Labeling Kit, Preparation of Biotin16-labeled DNA probes by PCR
Product Sizes
175 reactions
£324.00
APP-101-BIO16-175REACTIONS
About this Product
- SKU:
- APP-101-BIO16
- Application:
- PCR
- Conjugate:
- Biotin
- Extra Details:
- HighFidelity Biotin16 PCR Labeling Kit is designed to produce randomly Biotin16-modified DNA probes by PCR. Such probes are ideally suited for in situ hybridization and Northern Blot experiments. PCR-based labeling is superior to random-primed labeling by Klenow fragment if template amounts are limited or amplification of a specific DNA fragments is required. Biotin-16-dUTP is efficiently incorporated into DNA as substitute for its natural counterpart dTTP using an optimized reaction buffer and a High Fidelity Polymerase. 50 % Biotin-16-dUTP substitution typically results in an optimal balance between reaction and labeling efficiency. Individual optimization of Biotin-16-dUTP/dTTP ratio however, can easily be achieved with the single nucleotide format. The resulting Biotin16-modified DNA probe can subsequently be detected by HRP- or AP-modified Streptavidin. The kit contains sufficient reagents for 175 labeling reactions of 20 ul each (50 % Biotin16-dUTP substitution, 100 uM dATP/dGTP/dCTP, 50 uM dTTP, 50 uM Biotin16-dUTP).
- Shipping Conditions:
- Blue Ice
- Storage Conditions:
- -20[o]C Aliquot. Avoid freeze/thaw cycles.
- Supplier:
- Jena Bioscience
- Type:
- Assay Kits: Labelling