DIG 3'-End Oligonucleotide Labeling Kit with Digoxigenin-11-ddUTP
Product Sizes
25 reactions
APP-002-25REACTIONS
About this Product
- SKU:
- APP-002
- CE/IVD:
- RUO
- Extra Details:
- DIG 3'-End Oligonucleotide Labeling Kit with Digoxigenin-11-ddUTP contains all reagents (except oligonucleotide template to be labeled and materials for Digoxigenin detection) required for efficient 3'-End digoxigenylation of DNA oligonucleotides (length: 20 -100 bp, 5 pmol per reaction). The labeling principle is based on Terminal deoxynucleotidyl Transferase (TdT) that template-independently transfers 1 Digoxigenin-11-ddUTP to the 3'-OH group of ssDNA (e.g. an oligonucleotide) in the presence of CoCl2. It is similar to the principle of DIG Oligonucleotide 3'-End DNA Labeling Kit, 2nd Generation (Roche). The resulting 3'-End digoxigenylated oligonucleotides are ideally suited for applications involving sequence-specific protein binding or hybridiziation such as EMSA, Northern or Southern blots. Compared to internal, random digoxigenylated probes, Digoxigenin is located at the 3'-End only and less likely interferes with probe binding. TdT possesses a preference for single-stranded DNA (ssDNA) over dsDNA with 3'-overhangs or blunt ends. For the preparation of labeled dsDNA complexes, label each complementary oligonucleotide separately and anneal them before use.
- Shipping Conditions:
- Blue Ice
- Storage Conditions:
- -20[o]C Aliquot. Avoid freeze/thaw cycles.
- Supplier:
- Jena Bioscience
- Type:
- Proteins, Peptides, Small Molecules & Other Biomolecules: Small Molecules