BIO 3'-End Oligonucleotide Labeling Kit with Biotin-11-UTP
Product Sizes
25 reactions
APP-001-25REACTIONS
About this Product
- SKU:
- APP-001
- CE/IVD:
- RUO
- Conjugate:
- Biotin
- Extra Details:
- BIO 3'-End Oligonucleotide Labeling Kit with Biotin-11-UTP contains all reagents (except oligonucleotide template to be labeled and materials for Biotin detection) required for efficient 3'-End biotinylation of DNA oligonucleotides (length: 20 -100 bp, 5 pmol per reaction). The labeling principle is based on Terminal deoxynucleotidyl Transferase (TdT) that template-independently transfers 1-3 Biotin-11-UTPs to the 3'-OH group of ssDNA (e.g. an oligonucleotide) in the presence of CoCl2. It is similar to the principle of Biotin 3'-End DNA Labeling Kit (Pierce/Thermo Scientific). The resulting 3'-End biotinylated oligonucleotides are ideally suited for applications involving sequence-specific protein binding or hybridiziation such as EMSA, Northern or Southern blots. Compared to internal, random biotinylated probes, Biotin is located at the 3'-End only and less likely interferes with probe binding. TdT possesses a preference for single-stranded DNA (ssDNA) over dsDNA with 3'-overhangs or blunt ends. For the preparation of labeled dsDNA complexes, label each complementary oligonucleotide separately and anneal them before use.
- Shipping Conditions:
- Blue Ice
- Storage Conditions:
- -20[o]C Aliquot. Avoid freeze/thaw cycles.
- Supplier:
- Jena Bioscience
- Type:
- Proteins, Peptides, Small Molecules & Other Biomolecules: Small Molecules