Mca-KPLGL-Dap(Dnp)-AR-NH2
About this Product
- SKU:
- PS-060
- Additional Names:
- Mca-Lys-Pro-Leu-Gly-Leu-Dap(Dnp)-AR-NH2, fluorogenic, FRET substrate, matrix metalloproteinases , 720710-69-0, Mca-KPLGL-Dap(Dnp)-AR-NH2, PS-060, PS060
- CAS Number:
- 720710-69-0
- Conjugate:
- MCA
- Extra Details:
- Mca-KPLGL-Dap(Dnp)-AR-NH2 is a broad spectrum fluorogenic FRET substrate suitable for matrix metalloproteinases including intestinal collagenase (MMP-1), neutrophil collagenase (MMP-8) collagenase 3 (MMP-13), gelatinase A (MMP-2), gelatinase B (MMP-9), matrilysin (MMP-7) and MMP-17. Mca-KPLGL-Dap(Dnp)-AR-NH2 is cleaved very poorly by neutrophil elastase, and not at all by trypsin. Mca-KPLGL-Dap(Dnp)-AR-NH2 was obtained by the addition of an N-terminal lysine to Mca-PLGL-Dap(Dnp)-AR-NH, (PS-040) which improves substrate properties. It contains the highly fluorescent 7-methoxycoumarin (Mca) group that is efficiently quenched by resonance energy transfer to the 2,4-dinitrophenyl (Dnp) group. Mca-KPLGL-Dap(Dnp)-AR-NH2 is fully water soluble and allows measurement of metalloproteinase activity in tissue culture conditions such as on the surface of cells.
- Immunogen:
- Matrix metalloproteases
- Molecular Weight:
- 1221.3
- Physical State:
- Freeze dried solid
- Purity:
- >95%
- References:
- <p>Neumann et al (2004) Characterization of Mca-Lys-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2, a fluorogenic substrate with increased specificity constants for collagenases and tumor necrosis factor converting enzyme. Anal. Biochem. <strong>328</strong>(2) 166 doi:10.1016/j.ab.2003.12.035 </p><p>Devy et al (2009) Selective inhibition of matrix metalloproteinase-14 blocks tumor growth, invasion, and angiogenesis. Cancer Res. <strong>69</strong>(4) 1517 doi:10.1158/0008-5472.CAN-08-3255 </p><p>Walsh and Damjanovski (2011) IGF-1 increases invasive potential of MCF 7 breast cancer cells and induces activation of latent TGF-β1 resulting in epithelial to mesenchymal transition. Cell. Commun. Signal <strong>9</strong> 10 https://doi.org/10.1186/1478-811X-9-10</p>
- Sequence:
- Mca-Lys-Pro-Leu-Gly-Leu-Dap(Dnp)-Ala-Arg-NH2
- Shipping Conditions:
- Blue Ice
- Storage Conditions:
- Store desiccated, frozen and in the dark
- Size:
- 1 mg
- Supplier:
- Isca Biochemicals
- Type:
- Proteins, Peptides, Small Molecules & Other Biomolecules: Peptides
- Solubility:
- Soluble as a stock solution in DMSO
- Formula:
- C55H80N16O16
