Mca-RPPGFSAFK(Dnp)-OH
About this Product
- SKU:
- PS-050
- Additional Names:
- 323577-36-2, Mca-RPPGFSAFK(Dnp)-OH, Mca-(Ala7,Lys(Dnp)9)-bradykinin, fluorescent substrate , endothelin-converting enzyme-1, ECE-1, zinc metallopeptidase, fluorescent 7-methoxycoumarin, Mca, 2,4-dinitrophenyl, Dnp) group, PS-050, PS050|Mca-(Ala7,Lys(Dnp)9)-bradykinin
- CAS Number:
- 323577-36-2
- Conjugate:
- MCA
- Extra Details:
- Mca-RPPGFSAFK(Dnp)-OH, also known as Mca-(Ala7,Lys(Dnp)9)-bradykinin is a very sensitive fluorescent substrate for endothelin-converting enzyme-1 (ECE-1), the membrane-bound zinc metallopeptidase that is related to neprilysin. Mca-RPPGFSAFK(Dnp)-OH can also be used for assaying ACE1, ACE2, BACE, cathepsin A, cathepsin X/Z, ECE-1, ECE-2, MMP-2, MMP9, neprilysin, insulinase and thermolysin (TLN), although it is not a substrate for MMP1. Mca-RPPGFSAFK(Dnp)-OH substrate contains a highly fluorescent 7-methoxycoumarin (Mca) group that is efficiently quenched by resonance energy transfer to the 2,4-dinitrophenyl (Dnp) group.
- Immunogen:
- Endothelin converting enzyme 1
- Molecular Weight:
- 1388.5
- Physical State:
- Freeze dried solid
- Purity:
- >95%
- References:
- <p>Johnson and Ahn (2000) Development of an internally quenched fluorescent substrate selective for endothelin-converting enzyme-1. Anal Biochem. <strong>286</strong>(1) 112 doi:10.1006/abio.2000.4772 </p><p>Bruce et al (2008) Hydrogen bond residue positioning in the 599-611 loop of thimet oligopeptidase is required for substrate selection. FEBS J. <strong>275</strong>(22) 5607 doi:10.1111/j.1742-4658.2008.06685.x </p><p>Nelson et al (2009) Reduction of β-Amyloid Levels by Novel Protein Kinase Cϵ Activators. J. Biol. Chem.<strong> 284</strong> 34514 doi: 10.1074/jbc.M109.016683</p>
- Sequence:
- Mca-Arg-Pro-Pro-Gly-Phe-Ser-Ala-Phe-Lys-(Dnp)
- Shipping Conditions:
- Blue Ice
- Storage Conditions:
- Store desiccated, frozen and in the dark
- Size:
- 1 mg
- Supplier:
- Isca Biochemicals
- Type:
- Proteins, Peptides, Small Molecules & Other Biomolecules: Peptides
- Solubility:
- Soluble in DMSO
- Formula:
- C66H81N15O19
