Mca-SEVNLDAEFR-K(Dnp)-RR-NH2
About this Product
- SKU:
- PS-020
- Additional Names:
- Mca-(Asn °,Leu ¹)-Amyloid B Beta/A4 Protein Precursor, (667-676)-Lys(Dnp)-Arg-Arg amide|Mca-SEVNLDAEFR-K(Dnp)-RR-NH2, 438625-61-7, SEVNLDAEFRKRR, Mca-(Asn °,Leu ¹)-Amyloid B Beta/A4 Protein Precursor, (667-676)-Lys(Dnp)-Arg-Arg amide, Beta-secretase 1, BACE-1, FRET substrate, Swedish Lys-Met/Asn-Leu mutation, fluorescent 7-methoxycoumarin, PS-020, PS020
- CAS Number:
- 438625-61-7
- Conjugate:
- MCA
- Extra Details:
- Mca-SEVNLDAEFR-K(Dnp)-RR-NH2, also knowm as Mca-(Asn °,Leu ¹)-Amyloid B Beta/A4 Protein Precursor, (667-676)-Lys(Dnp)-Arg-Arg amide, is a Beta-secretase 1 (BACE-1) peptide FRET substrate, containing the 'Swedish' Lys-Met/Asn-Leu mutation of the amyloid precursor protein (APP) B Beta-secretase cleavage site. Cleavage at -Leu-Asp- of Mca-SEVNLDAEFR-K(Dnp)-RR-NH2 liberates the highly fluorescent 7-methoxycoumarin (Mca) fragment from the proximity quenching effect of the 2,4-dinitrophenyl (Dnp) internal quencher resulting in a large and easily detectable increase in fluorescence intensity.
- Immunogen:
- Secretases
- Molecular Weight:
- 2001.1
- Physical State:
- Freeze dried solid
- Purity:
- >95%
- References:
- <p>Koike et al (1999) Thimet Oligopeptidase Cleaves the Full-Length Alzheimer Amyloid Precursor Protein at a β-Secretase Cleavage Site in COS Cells. J. Biochem. <strong>126</strong>(1) 235 https://doi.org/10.1093/oxfordjournals.jbchem.a022428 </p><p>Klaver et al (2010), Glycosaminoglycan-induced activation of the β-secretase (BACE1) of Alzheimer's disease. J. Neurochem. <strong>112</strong> 1552 doi:10.1111/j.1471-4159.2010.06571.x</p>
- Sequence:
- Mca-Ser-Glu-Val-Asn-Leu-Asp-Ala-Glu-Phe-Arg-Lys(Dnp)-Arg-Arg-NH2
- Shipping Conditions:
- Blue Ice
- Storage Conditions:
- Store desiccated, frozen and in the dark
- Size:
- 1 mg
- Supplier:
- Isca Biochemicals
- Type:
- Proteins, Peptides, Small Molecules & Other Biomolecules: Peptides
- Solubility:
- Soluble in DMSO
- Formula:
- C86H125N27O29
