Mca-PLGL-Dap(Dnp)-AR-NH2
About this Product
- SKU:
- PS-010
- Additional Names:
- Mca-Pro-Leu-Gly-Leu-Dap(Dnp)-Ala-Arg-NH2, 140430-53-1, Mca-PLGL-Dap(Dnp)-AR-NH2, FRET substrate, matrix metalloproteinase, MMP, PS-010, PS010
- CAS Number:
- 140430-53-1
- Extra Details:
- Mca-PLGL-Dap(Dnp)-AR-NH2 is a sensitive synthetic peptide FRET substrate for the determination of matrix metalloproteinase (MMP) activity. Protease cleavage at the -Gly-Leu- bond releases the highly fluorescent 7-methoxycoumarin (Mca) group from the influence of the 2,4-dinitrophenyl (Dnp) internal quencher, resulting in a 190 fold increase in fluorescence intensity. Mca-PLGL-Dap(Dnp)-AR-NH2 is suitable for use in continuous assays at enzyme concentrations comparable to those used with macromolecular substrates and also for the assessment of total matrix metalloproteinase in crude preparations.
- Immunogen:
- Matrix metalloproteases
- Molecular Weight:
- 1093.2
- Physical State:
- Freeze dried solid
- Purity:
- >95%
- References:
- <p>Knight et al (1992) A novel coumarin-labelled peptide for sensitive continuous assays of the matrix metalloproteinases. FEBS Lett. <strong>296</strong>(3) 263 doi:10.1016/0014-5793(92)80300-6 </p><p>López-Pelegrín et al (2013) A novel family of soluble minimal scaffolds provides structural insight into the catalytic domains of integral membrane metallopeptidases. J. Biol. Chem. <strong>288</strong>(29) 21279 doi:10.1074/jbc.M113.476580</p>
- Sequence:
- Mca-Pro-Leu-Gly-Leu-Dap(Dnp)-Ala-Arg-NH2
- Shipping Conditions:
- Blue Ice
- Storage Conditions:
- Store desiccated, frozen and in the dark
- Size:
- 1 mg
- Supplier:
- Isca Biochemicals
- Type:
- Proteins, Peptides, Small Molecules & Other Biomolecules: Peptides
- Solubility:
- Soluble in DMSO
- Formula:
- C49H68N14O15
