Kemptide
About this Product
- SKU:
- KS-030
- Additional Names:
- Kemptide, substrate, cAMP-dependent protein kinase, PKA, phosphorylation, pyruvate kinase, phosphate, acceptor peptide, LRRASLG, 65189-71-1, KS-030, KS030
- CAS Number:
- 65189-71-1
- Extra Details:
- Kemptide is a synthetic heptapeptide that acts as a specific substrate for cAMP-dependent protein kinase (PKA). Kemptide is derived from a phosphorylation sequence of pig liver pyruvate kinase, a natural PKA substrate and is a phosphate acceptor peptide that when used as a synthetic substrate for PKA has a Km of 16 uM. Kemptide has kinetics comparable to native protein substrates.
- Immunogen:
- Kinases
- Molecular Weight:
- 771.9
- Physical State:
- Freeze dried solid
- Purity:
- >95%
- References:
- <p>Giembycz and Diamond (1990) Evaluation of Kemptide, a synthetic serine containing heptapeptide, as a phosphate acceptor for the estimation of cyclic AMP-dependent protein kinase activity in respiratory tissues Biochem. Pharmacol.<strong> 39</strong>(2) 271 <a href="https://pubmed.ncbi.nlm.nih.gov/2154228/" target="_blank" rel="nofollow">PMID: 2154228</a></p><p>Galello et al (2010) Characterization of substrates that have a differential effect on Saccharomyces cerevisiae protein kinase A holoenzyme activation. J. Biol. Chem. <strong>28</strong>5(39) 29770<a href="https://pubmed.ncbi.nlm.nih.gov/20639203/" target="_blank" rel="nofollow"> PMID: 20639203</a></p><p>Mena-Ulecia et al (2014) Study of the Affinity between the Protein Kinase PKA and Peptide Substrates Derived from Kemptide Using Molecular Dynamics Simulations and MM/GBSA. PLoS ONE<strong> 9</strong>(10) e109639<a href="https://pubmed.ncbi.nlm.nih.gov/25275314/" target="_blank" rel="nofollow"> PMID: 25275314</a></p><p><a href="https://pubchem.ncbi.nlm.nih.gov/compound/Kemptide" target="_blank" rel="nofollow">PubChem - Kemptide </a><br></p>
- Sequence:
- H-Leu-Arg-Arg-Ala-Ser-Leu-Gly-OH
- Shipping Conditions:
- Blue Ice
- Storage Conditions:
- Store dark, frozen and desiccated
- Size:
- 5 mg
- Supplier:
- Isca Biochemicals
- Type:
- Proteins, Peptides, Small Molecules & Other Biomolecules: Peptides
- Solubility:
- Soluble in dilute acid
- Formula:
- C32H61N13O9
