MCA-AVLQSGFR-Lys(Dnp)-Lys-NH2
About this Product
- SKU:
- CV-070
- Additional Names:
- MCA-AVLQSGFR-Lys(Dnp)-Lys-NH2, FRET substrate, severe acute respiratory syndrome coronavirus main protease, SARS-CoV Mpro, AVLQSGFRK, 7-methoxycoumarin, Mca, 2,4-dinitrophenyl, fluorescence, cv-070, cv070, 932391-88-3, covid, covid-19, covid 19|SARS Main Protease FRET Substrate
- CAS Number:
- 932391-88-3
- Extra Details:
- MCA-AVLQSGFR-Lys(Dnp)-Lys-NH2 is a FRET substrate for the severe acute respiratory syndrome coronavirus main protease (SARS-CoV Mpro). The MCA-AVLQSGFR-Lys(Dnp)-Lys-NH2 substrate sequence is derived from residues P4-P5' of the SARS-CoV Mpro N-terminal autoprocessing site which has the sequence AVLQSGFRK. Enzmatic cleavage between Q4 and S5 of MCA-AVLQSGFR-Lys(Dnp)-Lys-NH2 releases the highly fluorescent 7-methoxycoumarin (Mca) group from the influence of the 2,4-dinitrophenyl (Dnp) internal quencher, resulting in a large increase in fluorescence intensity.
- Immunogen:
- Serine proteases,Antivirals
- Molecular Weight:
- 1514.7
- Physical State:
- Freeze dried solid
- Purity:
- >95%
- References:
- <p>Yang et al (2005) Design of Wide-Spectrum Inhibitors Targeting Coronavirus Main Proteases. PLoS Biol. <strong>3</strong>(10) e324 <a href="https://pubmed.ncbi.nlm.nih.gov/16128623/" target="_blank" rel="nofollow">PMID: 16128623</a></p><p>Zhao et al (2008). Structure of the Main Protease from a Global Infectious Human Coronavirus, HCoV-HKU1. J. Virol. <strong>82</strong>(17) 8647 <a href="https://pubmed.ncbi.nlm.nih.gov/18562531/" target="_blank" rel="nofollow">PMID: 18562531</a></p><p>Bahun et al (2021) Inhibition of the SARS-CoV-2 3CLpro main protease by plant polyphenols, Food Chemistry <strong>131594</strong> <a href="https://www.sciencedirect.com/science/article/pii/S0308814621026005" target="_blank" rel="nofollow">https://doi.org/10.1016/j.foodchem.2021.131594.</a></p>
- Sequence:
- Mca-Ala-Val-Leu-Gln-Ser-Gly-Phe-Arg-Lys(Dnp)-Lys-NH2
- Shipping Conditions:
- Blue Ice
- Storage Conditions:
- Store desiccated, frozen and in the dark
- Size:
- 1 mg
- Supplier:
- Isca Biochemicals
- Type:
- Proteins, Peptides, Small Molecules & Other Biomolecules: Peptides
- Solubility:
- Soluble as a stock solution in DMSO
- Formula:
- C69H99N19O15
