N-peptide
About this Product
- SKU:
- AM-185
- Additional Names:
- N-peptide, WEYIPNV, gram-negative, periplasmic chaperone protein chaperone SurA, peptidyl-prolyl isomerase, PPIase, outer membrane porins, OMP, bacteria, antibiotic resistance, AM-185, AM185
- Extra Details:
- N-peptide, WEYIPNV, is a heptapeptide selected from a phage library that binds the gram-negative, periplasmic chaperone protein chaperone SurA with micromolar affinity. N-peptide (WEYIPNV) recognition is conferred by the first peptidyl-prolyl isomerase (PPIase) domain of SurA and N-peptide and SurA bind to each other in a 1:1 ratio. SurA is involved in the proper folding of outer membrane porins (OMPs), which protect bacteria against toxins in the extracellular environment and novel SurA inhibitors have the potential to overcome antibiotic resistance. Rhodamine-labeled N-peptide WEYIPNV can be used for a competitive assay for inhibitors of SurA.
- Immunogen:
- Protein-protein interactions
- Molecular Weight:
- 920.03
- Physical State:
- Freeze dried solid
- Purity:
- >95%
- References:
- <p>Bitto and McKay (2003) The Periplasmic Molecular Chaperone Protein SurA Binds a Peptide Motif That Is Characteristic of Integral Outer Membrane Proteins. J.Biol.Chem. <strong>278</strong> 49316 doi: 10.1074/jbc.M308853200 </p><p>Xu et al (2007) The periplasmic bacterial molecular chaperone SurA adapts its structure to bind peptides in different conformations to assert a sequence preference for aromatic residues. J. Mol. Biol.<strong> 373</strong>(2) 367 doi: 10.1016/j.jmb.2007.07.069. PMID: 17825319; PMCID: PMC2040117 </p><p>Bell et al (2018) Identification of inhibitors of the E. coli chaperone SurA using in silico and in vitro techniques. Bioorg. Med. Chem. Lett. <strong>28</strong>(22) 3540 doi: 10.1016/j.bmcl.2018.09.034 PMID: 30301675.</p>
- Sequence:
- H-Trp-Glu-Tyr-Ile-Pro-Asn-Val-OH
- Shipping Conditions:
- Blue Ice
- Storage Conditions:
- We recommend storage desiccated, frozen and in the dark
- Size:
- 1 mg
- Supplier:
- Isca Biochemicals
- Type:
- Proteins, Peptides, Small Molecules & Other Biomolecules: Peptides
- Solubility:
- Soluble in water
- Formula:
- C45H61N9O12
