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Assay Kits & ELISAs

Fluorescent Hypochlorite Detection Kit

Product Sizes
1 kit
£601.00
FLOCL100-2-1KIT
100 tests
£663.96
FLOCL100-2-100TESTS
About this Product
SKU:
FLOCL100-2
Additional Names:
Cell Viability Assay
Application:
Flow Cytometry
CE/IVD:
RUO
Extra Details:
Two cell-permeable dyes hydroxyphenyl fluorescein (HPF) and aminophenyl fluorescein (APF) detect hypochlorite production in cells. Hypochlorite production is observed by increased fluorescence of APF loaded cells with no increase in HPF loaded cells., ICT
Shipping Conditions:
Blue Ice
Storage Conditions:
2-8[o]C
Type:
Assay Kits: Detection
Figure 1. Detection of Hypochlorite ( -OCl ) in neutrophils. Neutrophils were isolated from porcine blood, washed in Krebs-Ringers phosphate buffer (as described in step V 1. above) and seeded in glass bottom dishes. The cells were then loaded with APF or HPF (10 mM final) by incubation for 30 minutes at room temperature. The Dye-loaded neutrophils were stimulated with PMA (2ng/mL).
Figure 2. Fluorescence images were acquired before and 10 minutes after stimulation. Excitation: 488 nm emission: 505-550 nm barrier filters. Hypochlorite production can be detected with increase APF fluorescence and no increase in HPF fluorescence.