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Assay Kits & ELISAs

Fluorescent Lactate Detection Kit

Product Sizes
100 tests
£516.00
FLLACT100-2-100TESTS
About this Product
SKU:
FLLACT100-2
Additional Names:
Metabolic Assay
Application:
ELISA
CE/IVD:
RUO
Extra Details:
This kit provides a reliable, sensitive fluorometric method for the quantification of lactate in biological samples such as serum, plasma, urine, and tissue extracts. The assay plate can be incubated and read in 15-30 min., ICT
Shipping Conditions:
Blue Ice
Storage Conditions:
2-8[o]C
Type:
Assay Kits: Detection
Figure 1. Assay principle
Figure 2. Typical standard curve. A new curve must be generated each time the assay is run.
Figure 3. At reduced glutathione concentrations higher than 0.2 mM, detection reagent oxidation results from side chain reaction between GSH and HRP. This could cause aberrant readings. To minimize this interference it is recommended to add N-Ethylmaleimide (NEM) at 40 mM to the reaction (4 uM of lactate was used here).