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Assay Kits & ELISAs

Flow Cytometry Cytotoxicity Assay

Product Sizes
100 tests
£429.44
ACT100-2-100TESTS
About this Product
SKU:
ACT100-2
Additional Names:
Cell Viability Assay
Application:
Flow Cytometry
Extra Details:
A non-radioactive cytotoxicity assay allowing phenotyping of a heterogeneous target cell population and detection of cytolytic activity at the cellular level, using flow cytometry.
Shipping Conditions:
Blue Ice
Storage Conditions:
2-8[o]C
Type:
Assay Kits: Cell Viability
Figure 1. K562 cells were stained with CFSE analog. Lymphocytes were added at: effector (E)/ target (T) ratios of 25:1, 50:1, and 100:1. Following incubation, 7AAD (live/dead) stain was added and cells analyzed via flow cytometry. % Cytotoxicity can be determined by an increase of target cells (lower right quadrant) moving into upper right quadrant and calculated by (R1/(R1+R2))*100.
Figure 2. A linear increase indicates the assay is optimized and an E/T ratio will be optimal for the experiments.
Figure 3. Cytolytic activity by lymphocytes was analyzed by gating target cells (upper and lower right quadrants) R2 (see below) and overlaid in a histogram.
Figure 4. Gating strategy. See protocol for details.
Figure 5. Gating strategy. See protocol for details. Cytolytic activity can be calculated (upper right quadrant/(upper right quadrant + lower right quadrant)) X 100 (Fig 5a-c).