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Assay Kits & ELISAs

Red Fluorescent SR-FLICA[R] Caspase-9 (LEHD) Assay Kit

Product Sizes
25 tests
£285.00
960-25TESTS
About this Product
SKU:
960
Application:
Flow Cytometry
CE/IVD:
RUO
Extra Details:
This in vitro assay employs the red fluorescent inhibitor probe SR-LEHD-FMK to label active caspase-9 enzyme in living cells. Analyze samples using fluorescence microscopy, a fluorescence plate reader, or flow cytometry., ICT
Immunogen:
Caspase-9
Physical State:
Cell Culture
Purification:
Jurkat cells were treated with 1 uM staurosporine to induce caspase-9 activity (left), or a negative control (right) for 3 hours, washed twice, then incubated with ICT's red caspase-9 inhibitor probe,
Shipping Conditions:
Blue Ice
Storage Conditions:
2-8[o]C
Type:
Assay Kits: Cell Viability
Figure 1. Jurkat cells, grown in suspension, were incubated with 1 uM staurosporine for 3 hours at 37°C to induce apoptosis, and then labeled with SR-LEHD-FMK (cat. 960/961) for 60 minutes at 37°C. Slides were prepared and imaged using a fluorescence microscope with a broad band pass filter. On slide B, cells appear very bright red, indicating a high level of active caspase-9 in these apoptotic cells. Non-induced cells did not fluoresce (slide A). Data courtesy of Dr. Brian W. Lee, ICT.
Figure 2. Jurkat cells were exposed to two experimental conditions (A and B). Each condition was treated with either DMSO (negative) or staurosporine (apoptotic), then labeled with SR-LEHD-FMK (cat. 960/961). Samples were read in a fluorescence plate reader (550 nm excitation and 590 nm emission using a 570 nm cut-off filter). Staurosporine induced caspase-9 activity 3.3X in both conditions: Condition A (11.0 to 36.5) and Condition B (12.7 to 41.8). Data courtesy of Mrs. Tracy Murphy, ICT 8Y18.