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Assay Kits & ELISAs

Red Fluorescent SR-FLICA[R] Caspase-3/7 (DEVD) Assay Kit

Product Sizes
100 tests
£718.00
932-100TESTS
About this Product
SKU:
932
Application:
Flow Cytometry
CE/IVD:
RUO
Extra Details:
This in vitro assay employs the red fluorescent inhibitor probe SR-DEVD-FMK to label active caspase 3 and caspase 7 enzymes in living cells. Analyze the fluorescent signal using fluorescence microscopy, a fluorescence plate reader, or by flow cytometry., ICT
Immunogen:
Caspase-3/7
Physical State:
Cell Culture
Purification:
Mouse EL4 thymoma cells were cultured in the absence (A) or presence (B) of 100 ug mitomycin for 18 hours. The cells were harvested and stained with ICT's SR-DEVD-FMK inhibitor probe (red histograms)
Shipping Conditions:
Blue Ice
Storage Conditions:
2-8[o]C
Type:
Assay Kits: Cell Viability
Figure 1. Control Alzheimer's Disease (AD) macrophages and curcumin-treated AD macrophages were exposed to FITC-labeled AB Beta and stained with SR-DEVD-FMK (#931/932). Macrophages that engulf FITC-AB Beta fluoresce green (right). Caspase-positive cells fluoresce red (left). Essentially all of the untreated AD macrophages engulfed FITC-AB Beta and became apoptotic. Curcumin-treated AD macrophages engulfed FITC-AB Beta but did not become apoptotic. Data courtesy of Dr. Milan Fiala, UCLA 072205.