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Assay Kits & ELISAs

Basic Calcein AM Cell Viability Kit

Product Sizes
25-250 tests
£306.00
9153-25-250TESTS
About this Product
SKU:
9153
Application:
Flow Cytometry
CE/IVD:
RUO
Extra Details:
This kit allows researchers to easily and simultaneously differentiate between live and dead cells within a single sample. Samples can be analyzed using a flow cytometer, fluorescence plate reader, or fluorescent microscope., ICT
Immunogen:
Intracellular esterases
Physical State:
Cell Culture
Purification:
Microscopy Analysis of Live Jurkat Cells Stained with Calcein AM Untreated Jurkat suspension cells were stained with 1 uM Calcein AM for 60 minutes at 37°C to detect live cells. The majority of the ce
Shipping Conditions:
Blue Ice
Storage Conditions:
2-8[o]C, -20[o]C
Type:
Assay Kits: Cell Viability
Figure 1. Live cells exhibiting green fluorescence (right of histogram) are easily distinguished from dead cells (unstained, left of histogram). Jurkat cells were either mock-treated (A) or killed by exposure to 90% ethanol (B). Cells were stained with Calcein AM, then analyzed in a flow cytometer with a FL-1 99% attenuation filter. Most (93.0%) live cells stained positive with Calcein AM (A, right), while 0.0% of the dead cells stained positive (B, right). Data courtesy of Dr. K. Strandberg.
Figure 2. Mock-treated Jurkat suspension cells were stained with Calcein AM to detect live cells. The majority of the cells imaged were considered to be live and healthy. Panel A reveals green fluorescence-stained live cells. Panel B shows a corresponding differential interference contrast (DIC) image, which reveals cell morphology. Microscope images were obtained using a Nikon Eclipse 90i microscope with a Hamamatsu Flash 4.0 camera. Data courtesy of Dr. Kristi Strandberg (ICT 226:95).
Jurkat suspension cells were mock-treated (black) or killed by exposure to 90% ethanol (red). A mix of the live and dead cells is shown in gray. Cells were stained with 0, 1, and 10 uM Calcein AM to detect live cells and analyzed using a fluorescence plate reader. The fluorescence signal in live cells increased with increasing concentration of Calcein AM. Dead cells had little or no fluorescence. Data courtesy of Dr. Strandberg (ICT 227:1-2).