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Assay Kits & ELISAs

Red Fluorescent SR-FLICA[R] Caspase-8 (LETD) Assay Kit

Product Sizes
25 tests
£286.00
9149-25TESTS
About this Product
SKU:
9149
Application:
Flow Cytometry
CE/IVD:
RUO
Extra Details:
This in vitro assay employs the red fluorescent inhibitor probe SR-LETD-FMK to label active caspase-8 enzymes in living cells. Analyze samples using fluorescence microscopy, a fluorescence plate reader, or flow cytometry., ICT
Immunogen:
Caspase 8
Physical State:
Cell Culture
Purification:
Jurkat cells were grown in suspension to 4 x 105 cells/mL, then divided into two separate TC-flasks. One population, "Non-Induced", received a DMSO vehicle control (A). The other population, "Induced"
Shipping Conditions:
Blue Ice
Storage Conditions:
2-8[o]C
Type:
Assay Kits: Cell Viability
Figure 1. Suspension Jurtkat cells were treated with DMSO (control, A) or 1 uM staurosporine (B) for 4 hours at 37°C, then stained with SR-LETD-FMK (kit cat. 9149/9150) for 1 hour at 37°C. Cells were washed three times and slides prepared. Treated cells appear bright red, indicating a high level of caspase-8 activity (B). Few non-induced cells are red, indicating minimal caspase-8 activity (A, Non-Induced, left). Data courtesy of Mrs. Tracy Murphy, ICT (220:68, 121815).