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Assay Kits & ELISAs

Necrosis vs Apoptosis Assay Kit

Product Sizes
100-200 tests
£726.00
9148-100-200TESTS
About this Product
SKU:
9148
Application:
Flow Cytometry
CE/IVD:
RUO
Extra Details:
This kit simultaneously detects both cell death due to both apoptosis and necrosis. It can be used to assess the effects of novel therapeutic agents on cell health. Analyze the fluorescent signal using fluorescence microscopy or flow cytometry., ICT
Immunogen:
Caspases, GC-rich DNA
Physical State:
Cell Culture
Purification:
Jurkat suspension cells were exposed to 1 uM of staurosporine for 4 hours at 37°C to induce apoptosis. Cells were dually stained with the green fluorescent FAM-FLICA poly caspase probe to detect apopt
Shipping Conditions:
Blue Ice
Storage Conditions:
2-8[o]C
Type:
Assay Kits: Cell Viability
Figure 1. Using FAM-FLICA®, non-apoptotic cells (unstained, left of histogram) can easily be distinguished from apoptotic cells exhibiting green fluorescence (right of histogram). Jurkat cells were either treated with DMSO as control (A), or 1 uM staurosporine (B) to induce apoptosis. Cells were stained with FAM-FLICA® and analyzed using a flow cytometer in FL-1. Only 7% of non-induced cells (A, right) are apoptotic compared with 96.5% of the induced cells (B, right). (ICT 226:17-19.)
Figure 2. Using 7-AAD, live cells (unstained, left side of each histogram) can easily be distinguished from dead/membrane compromised cells exhibiting red fluorescence (right side of each histogram). Jurkat cells were either untreated (A), or treated with 90% ethanol for 60 seconds, then stained with 7-AAD, and analyzed using a flow cytometer in FL-3. Only 4% of untreated cells (A) are dead compared with 97.6% of the treated cells (B). Data courtesy of Dr. Kristi Strandberg, ICT 226:30-31.
Figure 3. Jurkat suspension cells were exposed to staurosporine, and then stained with FAM-FLICA® and 7-AAD. Cells in early stage apoptosis fluoresce green with FAM-FLICA®, cells in mid-to-late stage apoptosis fluoresce green with FAM-FLICA® and red with 7-AAD, and necrotic cells fluoresce red with 7-AAD. Panel B the corresponding DIC image. FAM-FLICA® and 7-AAD were imaged using a 470-490 nm excitation filter plus >520 nm long pass filter tandem. Data courtesy of Dr. Brian W. Lee (ICT 196:70).
Figure 4. Live, unstained cells do not fluoresce (LL, lower left). Early stage apoptotic cells fluoresce green with FAM-FLICA® (LR, lower right). Dually stained green and red fluorescing cells represent cells in mid to late stage apoptosis; these cells have active caspase enzymes and compromised cell membranes (UR, upper right). Necrotic cells fluoresce red (UL, upper left).
Figure 5. Jurkat cells were treated with DMSO or staurosporine to induce apoptosis, and then stained with FAM-FLICA® (FL-1) and 7-AAD (FL-3). Live cells (lower left, B and D). Early stage apoptotic cells (lower right, B and D). Mid to late stage apoptosis (upper right, B and D). Necrotic cells (upper left, B and D). See Figure 4 for key. Only 10.8% of non-induced cells were apoptotic (LR: 4.5% + UR: 6.3%), compared with 97.1% of induced cells (D; LR: 46.4% + UR: 50.7%). ICT 226:17-19).