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Assay Kits & ELISAs

Far-Red Fluorescent FLICA[R] 660 Poly (active) Caspase (VAD) Assay Kit

Product Sizes
25-50 tests
£330.00
9120-25-50TESTS
About this Product
SKU:
9120
Application:
Flow Cytometry
CE/IVD:
RUO
Extra Details:
This in vitro assay employs the far-red fluorescent inhibitor probe 660-VAD-FMK to label active caspase enzymes in living cells. Analyze the fluorescent signal using fluorescence microscopy or flow cytometry., ICT
Immunogen:
Poly caspases
Physical State:
Cell Culture
Purification:
To demonstrate the fluorescence properties and apoptosis detection capability of the new far-red fluorescent FLICA 660 Poly Caspase Assay, Jurkat cells were were treated with 1 uM staurosporine for 4
Shipping Conditions:
Blue Ice
Storage Conditions:
2-8[o]C
Type:
Assay Kits: Cell Viability
Figure 1. FLICA® 660 was reconstituted in DMSO, diluted in diH2O, and analyzed on a Molecular Devices Gemini XPS 96-well fluorescence plate reader. The excitation spectrum (grey) was generated using an emission of 760 nm. The emission spectrum (red) was generated using an excitation of 600 nm.
Figure 2. Jurkat cells were treated with a negative control (left) or staurosporine, an apoptosis inducing agent (right), then stained with 660-VAD-FMK (cat. 9120) for 1 hour. Cells were washed twice and read on an Accuri C6 flow cytometer. Treatment with the negative control induced caspase activity in only 8.2% of cells (left), whereas treatment with staurosporine induced caspase activity in 96.5% of cells (right). This is a ratio of 12:1. Data courtesy of Mrs. Tracy Murphy, ICT, 213:48.
The activity of caspase 8 in breast cancer MCF-7 cells following a 24 h exposure to the tested compounds (EDAG-1 and EDAG-8) and cisplatin (concentrations of 0.5 and 1 uM). The FAM-FLICA® Caspase-8 Assay Kit (Cat no 9120) and a flow cytometer were used in the experiment. Image from publication, CC-BY-4.0, PMID: 39063006.
The activity of caspase 8 in breast cancer MDA-MB-231 cells following a 24 h exposure to the tested compounds (EDAG-1 and EDAG-8) and cisplatin (concentrations of 0.5 and 1 uM). The FAM-FLICA® Caspase-8 Assay Kit (Cat no 9120) and a flow cytometer were used in the experiment. Image from publication, CC-BY-4.0, PMID: 39063006.