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Assay Kits & ELISAs

Red Fluorescent SR-VAD-OPH in vitro Poly Caspase Apoptosis Detection Reagent

Product Sizes
4 x 146 ug
£653.00
6357-4X146UG
About this Product
SKU:
6357
Application:
Flow Cytometry
CE/IVD:
RUO
Extra Details:
O-phenoxy (OPH) inhibitors form a stable covalent thioether adduct with the reactive -SH group of active caspase enzymes. SR-VAD-OPH poly caspase inhibitor can be used with a fluorescence microscope, fluorescent plate reader, or flow cytometry., ICT
Immunogen:
Apoptosis, Poly Caspases
Physical State:
Cell Culture, Tissue
Shipping Conditions:
Blue Ice
Storage Conditions:
2-8[o]C
Type:
Assay Kits: Cell Viability
Figure 1. Jurkat cells were treated with staurosporine (Positive, Induced, right histogram). Negative control cells were spiked with a DMSO vehicle control (Negative, Non-induced, left histogram). Cells were stained with SR-VAD-OPH for 1 hour, washed twice, and read on a flow cytometer. Only 8.0% of control cells displayed caspase activity, whereas staurosporine induced caspase activity in 90.3% of the experimental cells. This is a ratio of 11:1. Data courtesy of Ms. Tracy Hanson, ICT 092713.
Figure 2. Jurkat cells were treated with staurosporine to induce apoptosis, then labeled with SR-VAD-OPH for 60 minutes. Cells were washed twice and wet-mount slides were prepared. A grey DIC image (left) was taken, and red fluorescence (right) was detected using a band pass filter (excitation at 565 nm, emission at 590-600 nm). SR-VAD-OPH reveals complete caspase activation in the form of red fluorescence in all cells. Data courtesy of Ms. Tracy Hanson, ICT (ICT111809; SR_OPH_111_17_18).
Figure 3. Jurkat cells were treated with DMSO (left) or staurosporine (right), then labeled with SR-VAD-OPH, followed by Green Live/Dead Stain (Cat. 6342). Four populations of cells were detected: unstained live cells (lower left); red early apoptotic cells stained with SR-VAD-OPH (lower right); late apoptotic cells (upper right) are dually stained with SR-VAD-OPH and Green Live/Dead Stain; and green necrotic cells (upper left). Data courtesy of Tracy Hanson, ICT (ICT-202:97; 091015).