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Assay Kits & ELISAs

Green Fluorescent FAM-VAD-OPH II in vitro poly Caspase Apoptosis Detection Reagent

Product Sizes
4 x 58 ug
£653.00
6355-4X58UG
About this Product
SKU:
6355
Application:
Flow Cytometry
CE/IVD:
RUO
Extra Details:
O-phenoxy (OPH) inhibitors form a stable covalent thioether adduct with the reactive -SH group of active caspase enzymes. FAM-VAD-OPH II poly caspase inhibitor can be used with a fluorescence microscope, fluorescent plate reader, or flow cytometer., ICT
Immunogen:
Apoptosis, Poly Caspases
Physical State:
Cell Culture, Tissue
Purification:
Jurkat cells (T lymphocytes) were treated with DMSO as a negative control (left) or 1 uM staurosporine (right), an apoptosis inducer, for 3 hours at 37°C. FAM-VAD-OPH II Poly Caspase Inhibitor was add
Shipping Conditions:
Blue Ice
Storage Conditions:
2-8[o]C
Type:
Assay Kits: Cell Viability
Figure 1. Jurkat cells were treated with DMSO (left) or staurosporine (right), labeled with FAM-VAD-OPH II, washed, and stained with PI. Four cell populations are seen: unstained live cells do not fluoresce (lower left); early apoptotic cells fluoresce green with FAM- VAD-OPH II (lower right); late apoptotic cells (upper right) fluoresce green (active caspases) and red (permeabilized cell membrane); and necrotic cells fluoresce red (upper left). Data of Dr. M. Olin, University of Minnesota.