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Assay Kits & ELISAs

Conjugation-Ready HRP Maleimide

Product Sizes
5.0 mg
£290.00
6294-5.0MG
About this Product
SKU:
6294
CE/IVD:
RUO
Extra Details:
Uses maleimide-activated HRP to create stable protein-HRP conjugates. Allows control of the HRP to protein ratio. Avoids HRP inactivation seen with periodate oxidation methods and protein aggregation / precipitation seen with glutaraldehyde methods., ICT
Shipping Conditions:
Blue Ice
Storage Conditions:
2-8[o]C
Type:
Assay Kits: Labelling
Figure 1. HRP-Maleimide-conjugated to thiolated antibody. Our Conjugation-Ready HRP-Maleimide reagent (HRP-M) makes it easy for scientists to create their own detection conjugates. The maleimide is linked to the HRP, which facilitates a stable bond with sulfhydryl (SH) groups on the target antibody (shown) or other target protein.
Figure 2. Thiolation. Conjugation-Ready HRP Maleimide links to proteins through free SH groups, which often must be added to the protein using Traut's reagent prior to conjugation.
Figure 3. HRP Conjugate used in detection ELISA. HRP-conjugated antibodies are used in ELISAs to generate the signal in the assay. By directly conjugating the HRP to the specific top detection antibody (right), one layer of the sandwich can be eliminated, thereby greatly simplifying the development and optimization of the assay.
Figure 4. HRP Conjugate used in Western Blot. Conjugated antibodies are used in Western Blots to generate the signal.
Figure 5. HRP-Conjugated antibody. Conjugation-Ready HRP Maleimide makes it easy to create detection conjugates. The maleimide is linked to the HRP, which facilitates a stable bond with sulfhydryl groups on the target antibody (shown) or protein.