Skip to content

Basket

You currently have no items in your basket.

Total (excl. vat) £0.00
View basket & checkout
Antibodies

Rabbit Anti-Phospho-Tau (S214) Antibody [JE66-83], Recombinant Rabbit monoclonal Antibody

Product Sizes
100uL
HA721797-HA721797
About this Product
SKU:
HA721797
Application:
WB
Buffer:
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
translate.label.attr.clone:
JE66-83
Clonality:
Recombinant Rabbit monoclonal Antibody
Concentration:
1 mg/mL.
Control:
SH-SY5Y treated with 200nM Calyculin A and 1μM Okadaic Acid for 1 hour cell lysate.
Format:
Liquid
Host:
Rabbit
Immunogen:
Synthetic phosphopeptide corresponding to residues surrounding S214 of human Tau protein.
Isotype:
IgG
Molecular Weight:
Predicted band size: 79 kDa
Purification:
Protein A affinity purified.
Reactivities:
Human, Mouse, Rat
Shipping Conditions:
Blue Ice
Storage Conditions:
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Supplier:
Huabio
Western blot analysis of Phospho-Tau (S214) on different lysates with Rabbit anti-Phospho-Tau (S214) antibody (HA721797) at 1/1,000 dilution. Lane 1: SH-SY5Y cell lysate Lane 2: SH-SY5Y treated with 200nM Calyculin A and 1μM Okadaic Acid for 1 hour cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 79 kDa Observed band size: 40-55 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721797) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.