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Elastic Fiber Staining Kit;E.V.G Method

Product Sizes
50mL
£206.00
RA20132-50ML
About this Product
SKU:
RA20132
Extra Details:
Connective tissue; in a narrow sense; is composed of three types of fibers: collagen fibers; reticular fibers; and elastic fibers. Elastic fibers are mainly distributed in the human arterial walls; alveolar walls; and skin. They appear yellow and are highly refractile when fresh. Elastic fiber staining can reveal changes in elastic fibers in skin tissues; such as elastic nevus; granuloma annulare; and scleroderma. It is also used to display and assess lesions of the endocardium and arteries; to observe whether certain pathological changes are accompanied by proliferation or destruction of elastic fibers; and to identify tumor components such as elastic fibromas. After elastic fiber staining; elastic fiber balls within the tumor can be clearly observed. Commonly used elastic fiber staining methods include the Gomori aldehyde-fuchsin method; resorcin-fuchsin method; orcein method; Victoria blue method; and iron hematoxylin method. EnkiLife Elastic Fiber Staining Solution (E.V.G Method) belongs to the iron hematoxylin staining category. The staining principle is based on the fact that elastin and pre-elastin fibers are highly cross-linked via disulfide bonds. After oxidation by iodine; some disulfide bonds are converted into anionic sulfated derivatives; which are strongly basophilic and selectively absorb the basic dye hematoxylin in the staining solution. This product uses the most commonly used Van Gieson (VG) counterstaining method. After staining; thick fibers are deeply stained; while thin fibers are slightly less distinct. Elastic fibers appear black; collagen fibers red; and muscle fibers and erythrocytes yellow; providing a clear contrast.
Research Area:
Pathology
Shipping Conditions:
Ambient
Storage Conditions:
store at RT for 12 months; keep away from light
Type:
Assay Kits: Staining & Imaging