Von Kossa Calcium Staining Kit
Product Sizes
50mL
£169.00
RA20130-50ML
About this Product
- SKU:
- RA20130
- Extra Details:
- Calcium is abundant in the human body; forming the skeleton as a structural support. It also plays vital roles in secretion; transport; muscle contraction; and nerve conduction. Calcium exists in two forms: Ionic calcium – found in blood circulation (i.e.; blood calcium); Bound calcium – combined with proteins; carbonates; or phosphates and deposited in tissues. Apart from bones and teeth; calcium is normally present in all tissues and cells; usually not in solid form. However; under certain pathological conditions; calcium may precipitate and deposit in tissues as solid particles; a process known as pathological calcification. The deposited calcium salts are mainly calcium phosphate; followed by calcium carbonate. Calcium salts are usually mono-refringent; except for calcium oxalate; which is bi-refringent. In H&E staining; calcium generally appears purple-blue. Many dyes can form chelates with calcium; including Alizarin Red S; Purpurin; and Nuclear Fast Red. Alizarin Red S; an anthraquinone derivative and sodium salt of alizarin sulfonate; can chelate with calcium in calcium carbonate or phosphate to form an orange-red complex. Common calcium staining methods include the silver nitrate method and Alizarin Red S method. Von Kossa staining (silver method) is a metal substitution technique. When Von Kossa silver solution reacts with sections containing insoluble calcium salts; calcium is replaced by silver. Upon light exposure; the silver salt is reduced to black metallic silver; making this method suitable for high-throughput calcium staining in tissue sections.
- Research Area:
- Pathology
- Shipping Conditions:
- Blue Ice
- Storage Conditions:
- store at 2-8[o]C for 12 months; keep away from light
- Type:
- Assay Kits: Staining & Imaging
