Tartrate-Resistant Acid Phosphatase (TRAP) Staining Kit
Product Sizes
10mL
£194.00
RA20118-10ML
About this Product
- SKU:
- RA20118
- Extra Details:
- Acid phosphatase (ACP) is widely distributed in virtually all tissues; predominantly within lysosomes; and is therefore commonly used as a lysosomal marker enzyme. Extralysosomal ACP is also present in the endoplasmic reticulum and cytoplasm. ACP isoforms differ among species; the optimum pH is 4.5–5.5. Tartrate-resistant acid phosphatase (TRAP) is found in follicles of normal human alveolar macrophages and in leukemic spleen cells; but is not released into blood. Consequently; serum TRAP activity largely reflects osteoclast function; enabling assessment of osteoclastic status via blood measurement. EnkiLife TRAP Staining Solution uses naphthol AS-BI as substrate; under acidic pH; ACP hydrolyses the substrate to release phosphate and naphthol; which couples with a diazonium salt to yield a colored product localized in the cytoplasm. Cells whose ACP is resistant to tartrate exhibit a positive reaction. Suitable for fresh blood smears; cytospins; and frozen sections.
- Research Area:
- Pathology
- Shipping Conditions:
- Blue Ice
- Storage Conditions:
- store at -20[o]C for 6 months; keep away from light
- Type:
- Assay Kits: Staining & Imaging
