Alcian Blue-Periodic Acid-Schiff (AB-PAS) Staining Kit
Product Sizes
50mL
£181.00
RA20102-50ML
100mL
£234.00
RA20102-100ML
About this Product
- SKU:
- RA20102
- Extra Details:
- Glycogen staining is one of the routine procedures in pathology. In 1946 McManus first applied the periodic acid–Schiff (PAS) technique to demonstrate mucins. The method is commonly used to visualize glycogen and other polysaccharides; and it additionally reveals neutral mucosubstances and certain acidic materials. Combined Alcian Blue (also known as Alcian Blue 8GX or Ingrain Blue 1) and PAS staining permits the simultaneous identification of neutral and acidic mucins in the same tissue section. The protocol is widely employed as a comprehensive mucin-detection tool: sections are first stained with standard Alcian Blue (pH 2.5); followed by the PAS reaction. Alcian Blue stains sialomucins; sulfomucins and proteoglycans blue; whereas the PAS reaction imparts a deep red/magenta color to neutral mucins. Tissues and cells that contain both neutral and acidic mucins appear in various shades of purple because Alcian Blue and Schiff reagent can interact. A typical example is the goblet cells of the small intestine; which contain both neutral and sialomucins. The staining mechanism of Alcian Blue relies on its being a copper-containing phthalocyanin dye that carries a cationic charge. The dye forms insoluble complexes with anionic groups (carboxyl and sulfate) in tissue mucins. Positively charged salt linkages between the dye and negatively charged acidic groups of acid mucopolysaccharides produce the blue color. Subsequent PAS staining then allows three different mucosubstance components to be distinguished. EnkiLife Alcian Blue-Periodic Acid-Schiff (AB-PAS) Staining Solution contains Alcian Blue staining solution and Schiff reagent as core components. After the procedure; glycogen; neutral mucins and various glycoproteins appear magenta; while acidic mucins remain blue. The kit is intended for research use only and is not suitable for clinical diagnosis or any other application.
- Research Area:
- Pathology
- Shipping Conditions:
- Blue Ice
- Storage Conditions:
- store at 2-8[o]C for 12 months; keep away from light
- Type:
- Assay Kits: Staining & Imaging
