Alcian Blue Staining Kit;pH 0.4
Product Sizes
50mL
£172.00
RA20101-50ML
About this Product
- SKU:
- RA20101
- Extra Details:
- Alcian Blue; also known as Alcian Blue 8GX or Ingrain Blue 1; is a copper-containing phthalocyanin dye originally used in textile dyeing. This cationic dye binds to acidic groups; specifically; Alcian Blue forms insoluble complexes with anionic groups such as carboxyl and sulfate residues in tissues. The dye molecule consists of a central copper-containing phthalocyanine ring linked via thioether bonds to four isothiourea groups that confer a moderate basicity and a net cationic charge. At pH 2.5; carboxyl groups in tissues are ionized and carry a negative charge; forming electrostatic (salt) linkages with the cationic dye; consequently; acidic mucosubstances rich in carboxyl groups (sulfated and sialylated mucins) are stained. At pH < 1.0; sulfate groups are ionized and negatively charged; allowing Alcian Blue to bind strongly to sulfate-rich mucosubstances. Neutral mucins (e.g.; those in gastric mucosa and Brunner’s glands) do not react with Alcian Blue under any of these conditions. EnkiLife Alcian Blue Staining Solution (pH 0.4) exploits these pH-dependent binding properties to discriminate mucosubstance classes: pH 2.5: carboxyl (-COOH) groups stain strongly; sulfate-containing mucins stain weakly. pH 1.0: carboxyl groups are unstained; sulfate-containing mucins stain. pH 0.4: only strongly acidic sulfated mucosubstances are stained.
- Research Area:
- Pathology
- Shipping Conditions:
- Blue Ice
- Storage Conditions:
- store at 2-8[o]C for 6 months; keep away from light
- Type:
- Assay Kits: Staining & Imaging
