Alcian Blue Staining Kit;pH 1.0
Product Sizes
50mL
£162.00
RA20100-50ML
About this Product
- SKU:
- RA20100
- Extra Details:
- Alcian Blue; also known as Alcian Blue 8GX; is a copper-containing phthalocyanine dye originally used for textile fiber staining. This cationic dye binds to acidic groups; forming insoluble complexes with anionic groups such as carboxyl and sulfate groups in tissues. The structure of Alcian Blue consists of a central copper-containing phthalocyanine ring linked to four isothiouronium groups via thioether bonds. The isothiouronium groups are moderately basic; giving the dye a positive charge. At pH 2.5; carboxyl groups in tissues are ionized and carry a negative charge; forming electrostatic bonds with the positively charged dye; thus staining acidic mucosubstances such as sialomucins and sulfomucins. EnkiLife Alcian Blue Staining Solution (pH 1.0) utilizes different pH values to distinguish types of mucosubstances: At pH 2.5: carboxyl groups are well stained; sulfated mucins are poorly stained. At pH 1.0: sulfate groups are ionized and carry a negative charge; allowing selective staining of sulfated mucosubstances. Neutral mucins (e.g.; in gastric mucosa and Brunner’s glands) do not react with Alcian Blue.
- Research Area:
- Pathology
- Shipping Conditions:
- Blue Ice
- Storage Conditions:
- store at 2-8[o]C for 6 months; keep away from light
- Type:
- Assay Kits: Staining & Imaging
