Alcian Blue Staining Kit;pH 2.5
Product Sizes
50mL
£151.00
RA20099-50ML
About this Product
- SKU:
- RA20099
- Extra Details:
- Alcian Blue; also known as Alcian Blue 8GX or Alcian Blue GX; is a copper-containing phthalocyanine dye originally used for textile fiber staining. This cationic dye binds to acidic groups; forming insoluble complexes with anionic groups such as carboxyl and sulfate groups in tissues. The structure of Alcian Blue consists of a central copper-containing phthalocyanine ring linked to four isothiouronium groups via thioether bonds. The isothiouronium groups are moderately basic; giving the dye a positive charge. At pH 2.5; carboxyl groups in tissues are ionized and carry a negative charge; forming electrostatic bonds with the positively charged dye; thus staining acidic mucosubstances such as sialomucins and sulfomucins. At pH 1.0; sulfate groups are ionized and carry a negative charge; allowing staining of sulfated mucosubstances. EnkiLife Alcian Blue Staining Solution (pH 2.5); also known as standard Alcian Blue solution; uses different pH values to distinguish types of mucosubstances: At pH 1.0: carboxyl (COOH) groups are not stained; sulfate (OSO3H) groups are stained. At pH 2.5: carboxyl groups are well stained; sulfated mucins are poorly stained. Neutral mucins (e.g.; in gastric mucosa and Brunner’s glands) do not react with Alcian Blue.
- Research Area:
- Pathology
- Shipping Conditions:
- Blue Ice
- Storage Conditions:
- store at 2-8[o]C for 12 months; keep away from light
- Type:
- Assay Kits: Staining & Imaging
