Modified Safranin O-Fast Green Cartilage Staining Kit
Product Sizes
50mL
£146.00
RA20095-50ML
100mL
£169.00
RA20095-100ML
About this Product
- SKU:
- RA20095
- Extra Details:
- Cartilage tissue is composed of chondrocytes and cartilage matrix. Cartilage tissue and its surrounding perichondrium constitute cartilage. Based on the fiber components in the matrix; cartilage is classified into hyaline cartilage; elastic cartilage; and fibrocartilage. There are various cartilage staining methods; such as toluidine blue; Alcian blue; and Safranin O methods. The staining principle of EnkiLife Modified Safranin O-Fast Green Cartilage Staining Method is that basophilic cartilage binds to the basic dye Safranin O; appearing red; while acidic bone tissue binds to the acidic dye Fast Green; appearing green or blue. The contrast between red cartilage and green/blue bone tissue allows clear differentiation. Safranin O is a cationic dye that binds to polyanions. It stains cartilage by binding to anionic groups (e.g.; chondroitin sulfate or keratan sulfate) in proteoglycans. The intensity of Safranin O staining is approximately proportional to the concentration of anions; indirectly reflecting the content and distribution of proteoglycans in the matrix. When cartilage is damaged; glycoproteins are released; leading to uneven matrix composition; resulting in weak or no staining by Safranin O. Quantitative analysis of Safranin O-stained cartilage matrix can be performed using image analysis software. Fast Green binds to collagen fibers and is resistant to fading. Differentiation is critical in Safranin O-Fast Green staining: over-differentiation may lead to no staining; while under-differentiation may result in overly intense staining.
- Research Area:
- Pathology
- Shipping Conditions:
- Ambient
- Storage Conditions:
- store at RT for 12 months
- Type:
- Assay Kits: Staining & Imaging
