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Assay Kits & ELISAs

Citrate Antigen Retrieval Buffer (10×; pH 6.0)

Product Sizes
100mL
£132.00
PA10019-100ML
500mL
£204.00
PA10019-500ML
About this Product
SKU:
PA10019
Application:
Immunohistochemistry, Immunofluorescence
Extra Details:
After fixation with paraformaldehyde; formalin or other aldehyde-based fixatives; proteins become cross-linked and carboxymethylated; masking epitopes and causing false-negative or weak signals in immunostaining. These cross-links can be reversed under high temperature or enzymatic conditions; allowing proteins to regain their native conformation. This process—antigen retrieval (AR)—is essential for reliable IHC/IF results. Heat-induced epitope retrieval (HIER) or enzymatic retrieval can be selected according to tissue and antigen characteristics; and the retrieval buffer must be at an appropriate pH. This product is a 10× citrate antigen retrieval buffer (pH 6.0). Dilute 1:10 to obtain the working solution; suitable for heat-mediated antigen retrieval.
Research Area:
Pathology
Shipping Conditions:
Blue Ice
Storage Conditions:
store at RT for 12 months
Type:
Assay Kits: IHC Kits