Recombinant Human LAP (TGF-beta 1)
Product Sizes
10ug
£ POA
EPT048-10UG
About this Product
- SKU:
- EPT048
- Additional Names:
- Transforming Growth Factor Beta-1; TGF-Beta-1; Latency-Associated Peptide; LAP; TGFB1; TGFB
- Extra Details:
- Transforming Growth Factor β-1 (TGFβ-1) is a secreted protein which belongs to the TGF-β family. TGFβ-1 is abundantly expressed in bone; articular cartilage and chondrocytes and is increased in osteoarthritis (OA). TGFβ-1 performs many cellular functions; including the control of cell growth; cell proliferation; cell differentiation and apoptosis. The precursor is cleaved into a latency-associated peptide (LAP) and a mature TGFβ-1 peptide.Disulfide-linked homodimers of LAP and TGF-beta 1 remain non-covalently associated after secretion; forming the small latent TGF-beta 1 complex. Purified LAP is also capable of associating with active TGF-beta with high affinity; and can neutralize TGF-beta activity. Covalent linkage of LAP to one of three latent TGF-beta binding proteins (LTBPs) creates a large latent complex that may interact with the extracellular matrix. TGF-beta activation from latency is controlled both spatially and temporally; by multiple pathways that include actions of proteases such as plasmin and MMP9; and/or by thrombospondin 1 or selected integrins. Although different isoforms of TGF-beta are naturally associated with their own distinct LAPs; the TGF-beta 1 LAP is capable of complexing with; and inactivating; all other human TGF-beta isoforms and those of most other species. Mutations within the LAP are associated with Camurati-Engelmann disease; a rare sclerosing bone dysplasia characterized by inappropriate presence of active TGF-beta 1.
- Molecular Weight:
- 28.5 KDa
- Purity:
- Greater than 95% as determined by reducing SDS-PAGE.
- Shipping Conditions:
- Blue Ice
- Storage Conditions:
- Lyophilized protein should be stored at < -20[o]C; though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7[o]C for 2-7 days. Aliquots of reconstituted samples are stable at < -20[o]C for 3 months.
- Supplier:
- ELK Biotechnology
- Type:
- Proteins, Peptides, Small Molecules & Other Biomolecules: Recombinant Proteins
- Endotoxin Level:
- Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.
