Mini Sample Mouse CD23 (Receptor II for the Fc Region of Immunoglobulin E) ELISA Kit
Product Sizes
24 tests
£143.00
E-MSEL-M0121-24TESTS
48 tests
£302.00
E-MSEL-M0121-48TESTS
96 tests
£365.00
E-MSEL-M0121-96TESTS
About this Product
- SKU:
- E-MSEL-M0121
- Additional Names:
- FCER2,BLAST-2,CD23A,CLEC4J,FCE2,IGEBF,Fc epsilon RII Recommended name:Low affinity immunoglobulin epsilon Fc receptor Alternative names:Fc-epsilon-RII Lymphocyte IgE receptor
- Application:
- ELISA
- Extra Details:
- The low affinity receptor for IgE, Fc epsilon RII (designated CD23) is a member of subgroup II of the C-type (Ca2+-dependent) lectin superfamily (1-4). Mouse CD23 is a 45-49 kDa type II transmembrane glycoprotein that binds mouse, but not human, IgE (1-4). The longest isoform is 331 amino acids (aa) in length and contains a 23 aa cytoplasmic domain, a 26 aa transmembrane segment, and a 282 aa extracellular domain (ECD). The ECD C-type lectin domain binds both protein and carbohydrate, with separate sites for binding IgE, CD21, and beta 2 and alpha v integrins (2). Coiled-coil topography within a connecting stalk contributes to oligomerization, which increases IgE affinity (1, 2). Mouse CD23 shares 88% aa identity with rat CD23 and up to 57% aa identity with isoforms of human, equine, porcine and bovine CD23. Isoforms which vary in the cytoplasmic sequence include the "a" isoform, which begins with the sequence MEENEYS and is constitutively expressed by B cells, and the "b" isoform, which begins with MDTHHT and is induced by IL-4 on a variety of inflammatory cells, B cells and epithelia (2, 5-7). The isoforms differentially participate in IgE-mediated endocytosis and phagocytosis (2, 8-10). CD23b and two 309 aa mouse CD23b isoforms, lacking either exon 5 or 6, also display distinct endocytic properties on intestinal epithelia (6, 11). Several soluble forms of CD23 are mainly generated by metalloprotease (especially ADAM10) and cysteine protease digestion, although potentially soluble mouse isoforms have also been sequenced (12-14). Both soluble and membrane-bound forms of CD23 include the lectin domain and show bioactivity. CD23 binding to monocyte integrins results in oxidative product generation and proinflammatory cytokine release (15). On human, but not mouse, B cells, sCD23 induces IgE secretion by binding CD21 (2, 9). In both, secreted IgE will bind B cell membrane CD23, rendering it unavailable for cleavage, and thus shutting down IgE production (2).
- Format:
- Colorimetric method,ELISA,Sandwich
- Physical State:
- Other Biological Fluid, Plasma, Serum
- Range:
- 78.13-5000 pg/mL
- Reactivities:
- Mouse
- Sensitivity:
- 46.88 pg/mL
- Shipping Conditions:
- Blue Ice
- Specificity:
- Mouse
- Storage Conditions:
- 2-8[o]C
- Supplier:
- Elabscience
- Type:
- Assay Kits: ELISAs
- Manufacturer's Data Sheet:p-mini_sample_mouse_cd23_(receptor_ⅱ_for_the_fc_region_of_immunoglobulin_e)_elisa_kit-e_msel_m0121
