NAD+/NADH Colorimetric Assay Kit (WST-8)
Product Sizes
96 tests
£397.00
E-BC-K804-M-96TESTS
About this Product
- SKU:
- E-BC-K804-M
- Additional Names:
- NAD+/NADH
- Extra Details:
- Detect total content of NAD+ and NADH Ethanol generates acetaldehyde under the action of enzyme. Meanwhile, NAD+ is reduced to NADH, NADH, under the action of hydrogen transmitter, transfer electrons to WST-8 to produce the yellow product, which has a characteristic absorption peak at 450 nm. Therefore, the total content of NAD+ and NADH can be quantified by measure the OD value at 450 nm. Detect NADH After treating sample, heat at 60oC water bath for 30 min. the NAD+ of the sample is decomposed and only NADH remains. NADH reduces WST-8 to form yellow product, and the amount of NADH is determined by measure the OD value at 450 nm. Detect NAD+ and NAD+/NADH The content of NAD+ and the ratio of NAD+/NADH in the sample can be obtained according to the total content of NAD+ and NADH obtained of the first two steps as well as the separate content of NADPH. Note: NADP+ and NADPH have no effect on the determination results.
- Format:
- Colorimetric
- Physical State:
- Tissue
- Range:
- 0.02-5.0 umol/L
- Sensitivity:
- 0.02 umol/L
- Shipping Conditions:
- Blue Ice
- Storage Conditions:
- -20[o]C Avoid freeze/thaw cycles. Protect from light.
- Supplier:
- Elabscience
- Type:
- Assay Kits: Detection
- Manufacturer's Data Sheet:nadh_colorimetric_assay_kit_(wst_8)-e_bc_k804_m
