NADP+/NADPH Colorimetric Assay Kit (WST-8)
Product Sizes
96 tests
£378.00
E-BC-K803-M-96TESTS
About this Product
- SKU:
- E-BC-K803-M
- Additional Names:
- NADP;NADPH
- Extra Details:
- Detect total content of NADP+ and NADPH: Glucose 6-phosphate (G6P) is oxidized to 6-phosphate gluconolactone (6-PG) by glucose-6-phosphate dehydrogenase (G6PDH), and NADP+ is reduced to NADPH during this reaction. NADPH, under the action of 1-mPMS, transfer electrons to WST-8 to produce the yellow product, which has a characteristic absorption peak at 450 nm. Therefore, the total content of NADP+ and NADPH can be quantified by measure the OD value at 450 nm. Detect NADPH: After treating sample, heat at 60°C water bath for 30 min. the NADP+ of the sample is decomposed and only NADPH remains. NADPH reduces WST-8 to form Formazan, and the amount of NADPH is determined by measure the OD value at 450 nm. Detect NADP+ and NADP+/NADPH: The content of NADP+ and the ratio of NADP+/NADPH in the sample can be obtained according to the total content of NADP+ and NADPH obtained of the first two steps as well as the separate content of NADPH.
- Format:
- Colorimetric
- Physical State:
- Tissue
- Range:
- 0.02-5.0 umol/L
- Sensitivity:
- 0.02 umol/L
- Shipping Conditions:
- Blue Ice
- Storage Conditions:
- -20[o]C Avoid freeze/thaw cycles. Protect from light.
- Supplier:
- Elabscience
- Type:
- Assay Kits: Detection
- Manufacturer's Data Sheet:nadph_colorimetric_assay_kit_(wst_8)-e_bc_k803_m
