NADP-Malic Enzyme (NADP-ME) Activity Colorimetric Assay Kit
Product Sizes
48 tests
£157.00
E-BC-K555-M-48TESTS
96 tests
£205.00
E-BC-K555-M-96TESTS
About this Product
- SKU:
- E-BC-K555-M
- Additional Names:
- NADP-ME
- Extra Details:
- Malic enzyme (ME) is widely present in eukaryotes and prokaryotes and participates in multiple metabolic pathways. Its main metabolic substrate is malic acid, which mainly catalyzes the oxidation and decarboxylation of malic acid to pyruvate and CO2, accompanied by the reduction of NAD(P)+ to NAD(P)H. Malic acid is an important intermediate product in the tricarboxylic acid cycle. Under the catalysis of ME, malic acid is converted into pyruvate and NAD(P)H to regulate cellular energy, REDOX balance and the synthesis of biological macromolecules. In mammalian cells, malic enzymes mainly consist of NADP+ dependent ME1 in the cytoplasm, NAD+ dependent ME2 in the mitochondria, and NADP+ dependent ME3 in the mitochondria. Inhibiting ME1 can induce cellular senescence or apoptosis to suppress the growth of tumor cells. The detection principle of this kit: The enzyme catalyzes the substrate reaction to generate NADPH, which has a characteristic absorption peak at 340 nm. The activity of NADP-ME can be determined by measuring the rate of increase in absorbance at 340 nm.
- Format:
- Colorimetric
- Immunogen:
- NADP-ME
- Physical State:
- Tissue
- Range:
- 2.68-338.96 U/L
- Sensitivity:
- 2.68 U/L
- Shipping Conditions:
- Blue Ice
- Storage Conditions:
- -20[o]C Avoid freeze/thaw cycles. Protect from light.
- Supplier:
- Elabscience
- Type:
- Assay Kits: Detection
- Manufacturer's Data Sheet:p-nadp_malic_enzyme_(nadp_me)_activity_colorimetric_assay_kit-e_bc_k555_m
