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Assay Kits & ELISAs

Fluo-4 Calcium Fluorometric Assay Kit

Product Sizes
96 tests
£374.00
E-BC-F100-96TESTS
About this Product
SKU:
E-BC-F100
Extra Details:
As an important metal ion in biological organism, calcium ion not only participates in the transmission of intracellular signals, but also plays an important role in many life activities of cells. Therefore, the detection of calcium ion concentration is of great significance in experimental research. This assay kit utilizes the cell-membrane-permeable calcium fluorescent probe Fluo-4 AM. The probe is non-fluorescent outside cells. Once inside cells, it is hydrolyzed by intracellular esterases to Fluo-4, which becomes trapped within the cytoplasm. Free Fluo-4 exhibits very weak fluorescence, but its fluorescence intensity increases significantly upon binding to Ca2+ . The probe shows maximum excitation at approximately 490 nm and maximum emission at 525 nm, and the fluorescence intensity is directly proportional to the intracellular calcium level.
Format:
Fluorometric method
Immunogen:
Ca
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles. Protect from light.
Supplier:
Elabscience
Type:
Assay Kits: Detection