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Antibodies

Purified Anti-Human/Monkey CD20 Antibody[2H7], Functional Grade

Product Sizes
100 ug
£89.00
E-AB-F12120-100UG
1 mg
£171.00
E-AB-F12120-1MG
5 mg
£490.00
E-AB-F12120-5MG
25 mg
£1515.00
E-AB-F12120-25MG
50 mg
£2247.00
E-AB-F12120-50MG
100 mg
£3211.00
E-AB-F12120-100MG
About this Product
SKU:
E-AB-F12120
Additional Names:
B-lymphocyte surface antigen B1,Bp35,Leukocyte surface antigen Leu-16,MS4A1
Application:
Depletion, Flow Cytometry
Buffer:
Sterile PBS, pH 7.2. < 1.0 EU per mg of the antibody as determined by the LAL method.
translate.label.attr.clone:
2H7
Clonality:
Monoclonal
Concentration:
>= 1 mg/ml
Extra Details:
CD20 is a 33-37 kD, four transmembrane spanning protein, also known as B1 and Bp35. CD20 is expressed on pre-B-cells, resting and activated B cells (not plasma cells), some follicular dendritic cells, and at low levels on a T cell subset. CD20 is heavily phosphorylated on activated B cells and malignant B cells. Homo-oligomeric complexes of CD20 are thought to form Ca2+ conductive ion channels in the plasma membrane of B cells. The CD20 molecule is involved in B-cell activation and is associated with various Src family kinases (Lyn, Lck, Fyn). It exists in a complex with MHC class I and II, CD53, CD81, and CD82. None (Azide-Free,Low Endotoxin) are perfectly suited to be used in culture or in vivo (for nonhuman studies) for functional assays blocking, neutralizing, activation or depletion where the presence of azide may damage cells or exogenous endotoxin may signal or activate cells.
Host:
Mouse
Immunogen:
Recombinant Human CD20 protein
Isotype:
IgG2b, kappa
Purity:
>98%
Purification:
protein a/g purified
Reactivities:
Human, Non-human Primate
Shipping Conditions:
Blue Ice
Storage Conditions:
2-8[o]C Avoid freeze/thaw cycles., -20[o]C Avoid freeze/thaw cycles.
Supplier:
Elabscience
Type:
Antibody: Monoclonal Antibody
Human peripheral blood lymphocytes were stained with 0.2 ug Purified Anti-Human CD20 Antibody[2H7], Functional Grade (Right) and 0.2 ug Mouse IgG2b, K Kappa Isotype Control (Left), followed by Elab Fluor® 647-conjugated Goat Anti-Mouse IgG Secondary Antibody, then anti-Human CD19 FITC-conjugated Monoclonal Antibody.
HEK293T cells transfected with pcDNA3.1 plasmid encoding Human CD154 gene were stained with 0.2 ug Purified Anti-Human CD154 Antibody[5C8] (Right) and 0.2 ug Mouse IgG2a, K Kappa Isotype Control (Left), followed by APC-conjugated Goat Anti-Mouse IgG Secondary Antibody.
Human peripheral blood lymphocytes were stained with 0.2 ug Purified Anti-Human CD19 Antibody[4G7] (Right) and 0.2 ug Mouse IgG1, K Kappa Isotype Control (Left), followed by APC-conjugated Goat Anti-Mouse IgG Secondary Antibody, then anti-Human CD22 PE-conjugated Monoclonal Antibody.
Human peripheral blood lymphocytes were stained with 0.2 ug AF/LE Purified Anti-Human HLA-A,B,C Antibody[W6/32] (Right) and 0.2 ug Mouse IgG2a, K Kappa Isotype Control (Left), followed by Elab Fluor® 647-conjugated Goat Anti-Mouse IgG Secondary Antibody.
Molt-4 were activated for 4 hour with MIX, then stained with 0.2 ug AF/LE Purified Anti-Human CD279 Antibody[J116] (Right) and 0.2 ug Mouse IgG1, K Kappa Isotype Control(Left), followed by APC-conjugated Goat Anti-Mouse IgG Secondary Antibody.