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Antibodies

Purified Anti-Mouse F4/80 Antibody[CI:A3-1], Functional Grade

Product Sizes
100 ug
£89.00
E-AB-F09950-100UG
1 mg
£171.00
E-AB-F09950-1MG
5 mg
£490.00
E-AB-F09950-5MG
25 mg
£1515.00
E-AB-F09950-25MG
50 mg
£2247.00
E-AB-F09950-50MG
100 mg
£3211.00
E-AB-F09950-100MG
About this Product
SKU:
E-AB-F09950
Additional Names:
Gpf480,Adgre1,Adhesion G protein-coupled receptor E1,Cell surface glycoprotein F4/80,EGF-like module receptor 1,Emr1
Application:
Depletion, Flow Cytometry
Buffer:
Sterile PBS, pH 7.2. < 1.0 EU per mg of the antibody as determined by the LAL method.
translate.label.attr.clone:
CI:A3-1
Clonality:
Monoclonal
Concentration:
>= 1 mg/ml
Extra Details:
F4/80 is a 160 kD glycoprotein. It is characterized as a member of the epidermal growth factor (EGF)-transmembrane 7 (TM7) family. F4/80, also known as EMR1 or Ly71, has been widely used as a murine macrophage marker, which is expressed on the majority of tissue macrophages including peritoneal macrophages, macrophages in lung, gut, thymus and red pulp of spleen (but not on the macrophages located in T cell areas of the spleen, lymph node and Peyer's patch), Kuffer cells, Langerhans cells, and bone marrow stromal cells. F4/80 has also been shown on a subset of dendritic cells. The biological ligand of F4/80 has not been identified, but it has been reported that F4/80 is required for induction of CD8+ T cells-mediated peripheral tolerance. None (Azide-Free,Low Endotoxin) are perfectly suited to be used in culture or in vivo (for nonhuman studies) for functional assays blocking, neutralizing, activation or depletion where the presence of azide may damage cells or exogenous endotoxin may signal or activate cells.
Host:
Rat
Immunogen:
Recombinant Mouse F4/80 protein
Isotype:
IgG2b, kappa
Purity:
>98%
Purification:
protein a/g purified
Reactivities:
Mouse
Shipping Conditions:
Blue Ice
Storage Conditions:
2-8[o]C Avoid freeze/thaw cycles., -20[o]C Avoid freeze/thaw cycles.
Supplier:
Elabscience
Type:
Antibody: Monoclonal Antibody
Mouse peritoneal macrophages were stained with 0.2 ug Purified Anti-Mouse F4/80 Antibody[CI:A3-1], Functional Grade (Right) and 0.2 ug Rat IgG2b, K Kappa Isotype Control (Left), followed by Elab Fluor® 647-conjugated Goat Anti-Rat IgG Secondary Antibody.
C57/BL6 Mouse splenocytes were stained with 0.2 ug Purified Anti-Mouse CD45 Antibody[M1/9.3.4.HL.3] (Right) and 0.2 ug Rat IgG2a, K Kappa Isotype Control (Left), followed by Elab Fluor® 647-conjugated Goat Anti-Rat IgG Secondary Antibody.
MCF-7 were stained with 0.2 ug Purified Anti-Human CD227 Antibody[AR20.5] (Right) and 0.2 ug Mouse IgG1, K Kappa Isotype Control (Left), followed by Elab Fluor® 647-conjugated Goat Anti-Mouse IgG Secondary Antibody.
Ht29 were stained with 0.2 ug Purified Anti-Human CD326 Antibody[rMOC-31] (Right) and 0.2 ug Mouse IgG1, K Kappa Isotype Control (Left), followed by Elab Fluor® 647-conjugated Goat Anti-Mouse IgG Secondary Antibody.
HEK293T cells transfected with pcDNA3.1 plasmid encoding Human IFN-G Gamma gene were stained with 0.2 ug Purified Anti-Human IFN-G Gamma Antibody[4S.B3] (Right) and 0.2 ug Mouse IgG1, K Kappa Isotype Control (Left), followed by Elab Fluor® 647-conjugated Goat Anti-Mouse IgG Secondary Antibody.