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Antibodies

Purified Anti-Mouse MHC II (I-A/I-E) Antibody[M5/114], Functional Grade

Product Sizes
100 ug
£89.00
E-AB-F09900-100UG
1 mg
£171.00
E-AB-F09900-1MG
5 mg
£490.00
E-AB-F09900-5MG
25 mg
£1515.00
E-AB-F09900-25MG
50 mg
£2247.00
E-AB-F09900-50MG
100 mg
£3211.00
E-AB-F09900-100MG
About this Product
SKU:
E-AB-F09900
Additional Names:
H2-Ab1/Eb1,I-E beta MHC class II,MHC class II,MHC class II H2-IA-beta-psi,Major histocompatibility protein class II beta chain
Application:
Block/Inhibit/Neutralise, Cell-based/Functional Assay, Flow Cytometry
Buffer:
Sterile PBS, pH 7.2. < 1.0 EU per mg of the antibody as determined by the LAL method.
translate.label.attr.clone:
M5/114
Clonality:
Monoclonal
Concentration:
>= 1 mg/ml
Extra Details:
These class II molecules are expressed on antigen presenting cells (including B cells) and a subset of T cells from H-2b,d,q,r bearing mice and are involved in antigen presentation to T cells expressing CD3/TCR and CD4 proteins. None (Azide-Free,Low Endotoxin) are perfectly suited to be used in culture or in vivo (for nonhuman studies) for functional assays blocking, neutralizing, activation or depletion where the presence of azide may damage cells or exogenous endotoxin may signal or activate cells.
Host:
Rat
Immunogen:
Recombinant Mouse MHC class II protein
Isotype:
IgG2b, kappa
Purity:
>98%
Purification:
protein a/g purified
Reactivities:
Mouse
Shipping Conditions:
Blue Ice
Storage Conditions:
2-8[o]C Avoid freeze/thaw cycles., -20[o]C Avoid freeze/thaw cycles.
Supplier:
Elabscience
Type:
Antibody: Monoclonal Antibody
C57/BL6 Mouse splenocytes were stained with 0.2 ug Purified Anti-Mouse MHC II (I-A/I-E) Antibody[M5/114], Functional Grade (Right) and 0.2 ug Rat IgG2b, K Kappa Isotype Control (Left), followed by Elab Fluor® 647-conjugated Goat Anti-Rat IgG Secondary Antibody.
Human peripheral blood lymphocytes were stained with 0.2 ug Purified Anti-Human TCRVB Beta1 Antibody[BL37.2] (Right) and 0.2 ug Rat IgG1, K Kappa Isotype Control (Left), followed by Elab Fluor® 647-conjugated Goat Anti-Rat IgG Secondary Antibody, then anti-Human CD3 PE-conjugated Monoclonal Antibody.
Human peripheral blood lymphocytes were stained with 0.2 ug Purified Anti-Human TCRVB Beta7.1 Antibody[ZOE5.1] (Right) and 0.2 ug Mouse IgG2a, K Kappa Isotype Control (Left), followed by Elab Fluor® 647-conjugated Goat Anti-Mouse IgG Secondary Antibody, then anti-Human CD3 PE-conjugated Monoclonal Antibody.
Human peripheral blood lymphocytes were stained with 0.2 ug Purified Anti-Human TRAV5 Antibody[MH3-2] (Right) and 0.2 ug Mouse IgG2a, K Kappa Isotype Control (Left), followed by Elab Fluor® 647-conjugated Goat Anti-Mouse IgG Secondary Antibody, then anti-Human CD3 PE-conjugated Monoclonal Antibody.
Human peripheral blood lymphocytes were stained with 0.2ug Purified Anti-Human TRAV12-1 Antibody[S511] (Right) and 0.2ug mouse IgG2b,K Kappa lsotype Control (Left), followed by Elab Fluor® 647-conjugated goat Anti-mouse IgG Secondary Antibody, then anti-human CD3 PE-conjugated Monoclonal Antibody.