Recombinant HIF1 beta Monoclonal Antibody
Product Sizes
50 ul
£198.00
E-AB-81565-50UL
100 ul
£291.00
E-AB-81565-100UL
About this Product
- SKU:
- E-AB-81565
- Additional Names:
- ARNT;ARNT protein;Aryl hydrocarbon receptor nuclear translocator;Class E basic helix-loop-helix protein 2;Dioxin receptor;Dioxin receptor nuclear translocator;Drnt;HIF 1 beta;HIF 1beta;HIF-1-beta;HIF1-beta;HIF1B;HIF1beta;Hypoxia Inducible Fact;arnT;bHLHe2
- Application:
- IHC-Paraffin, Immunohistochemistry, Western Blot
- Buffer:
- 50mm tris-glycine
- translate.label.attr.clone:
- R05-3B2
- Clonality:
- Monoclonal
- Concentration:
- 300 ug/ml
- Extra Details:
- Hypoxia-inducible factor 1 (HIF1) is a heterodimeric transcription factor that plays a critical role in the cellular response to hypoxia (1). The HIF1 complex consists of two subunits, HIF-1A Alpha and HIF-1B Beta, which are basic helix-loop-helix proteins of the PAS (Per, ARNT, Sim) family (2). HIF1 regulates the transcription of a broad range of genes that facilitate responses to the hypoxic environment, including genes regulating angiogenesis, erythropoiesis, cell cycle, metabolism and apoptosis. The widely expressed HIF-1A Alpha is typically degraded rapidly in normoxic cells by the ubiquitin/proteasomal pathway. Under normoxic conditions, HIF-1A Alpha is proline hydroxylated leading to a conformational change that promotes binding to the von Hippel Lindau protein (VLH) E3 ligase complex; ubiquitination and proteasomal degradation follows (3,4). Both hypoxic conditions and chemical hydroxylase inhibitors (such as desferrioxamine and cobalt) inhibit HIF-1A Alpha degradation and lead to its stabilization. In addition, HIF-1A Alpha can be induced in an oxygen-independent manner by various cytokines through the PI3K-AKT-mTOR pathway (5-7).HIF-1B Beta is also known as AhR nuclear translocator (ARNT) due to its ability to partner with the aryl hydrocarbon receptor (AhR) to form a heterodimeric transcription factor complex (8). Together with AhR, HIF-1B Beta plays an important role in xenobiotics metabolism (8).
- Host:
- Rabbit
- Immunogen:
- Recombinant protein of human HIF1 beta
- Isotype:
- IgG
- Purification:
- Affinity Purified
- Reactivities:
- Hamster, Human, Mouse, Rat
- Shipping Conditions:
- Blue Ice
- Storage Conditions:
- -20[o]C Avoid freeze/thaw cycles.
- Supplier:
- Elabscience
- Type:
- Antibody: Monoclonal Antibody
- Manufacturer's Data Sheet:p-recombinant_hif1_beta_monoclonal_antibody-e_ab_81565
