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Antibodies

(KO Validated) Vimentin Polyclonal Antibody

Product Sizes
60 ul
£254.00
E-AB-63601-60UL
120 ul
£383.00
E-AB-63601-120UL
200 ul
£587.00
E-AB-63601-200UL
About this Product
SKU:
E-AB-63601
Additional Names:
CTRCT30,HEL113,VIM,Vimentin,vimentin
Application:
ELISA, IHC-Paraffin, Immunofluorescence, Immunohistochemistry, Western Blot
Buffer:
Phosphate buffered solution, pH 7.4, containing 0.05% stabilizer and 50% glycerol.
Clonality:
Polyclonal
Concentration:
1 mg/ml
Extra Details:
This gene encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in this gene causes a dominant, pulverulent cataract.
Host:
Rabbit
Immunogen:
Recombinant fusion protein of human Vimentin (NP_003371.2).
Isotype:
IgG
Purification:
Affinity Purified
Reactivities:
Human, Mouse, Rat
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Supplier:
Elabscience
Type:
Antibody: Polyclonal Antibody
Immunofluorescence analysis of Mouse kidney tissue using Vimentin Rabbit pAb (A11952SP) at a dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining.
Western blot analysis of extracts from normal (control) and Vimentin knockout (KO) 293T cells using Vimentin Polyclonal Antibody at dilution of 1:1000.
Immunohistochemistry of paraffin-embedded Human tonsil using Vimentin Polyclonal Antibody at dilution of 1:150 (40x lens).
Immunohistochemistry of paraffin-embedded Human stomach using Vimentin Polyclonal Antibody at dilution of 1:150 (40x lens).
Immunofluorescence analysis of L929 cells using Vimentin Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.